Two such sets were prepared: one for inoculating with test strain (obtained from sputum samples) and the other for standard H37Rv strain. For each isolate, (originally from the sputum samples) L-J medium slants containing ethambutol, in serially doubling concentration ranging from 1mcg/ml to 16 mcg/ml were prepared. "Resistance ratio" is expressed as the ratio of the MIC of the test strain to the MIC of the standard H37Rv strain of M. Method: From sputum samples of proved cases of pulmonary tuberculosis, who had not received any antitubercular drug earlier in their life time, the acid-fast bacilli were cultured and speciated by colony morphology, time taken to grow, and no growth on PNBA medium, niacin and nitrate reduction test positive were identified as M. ethambutol and to determine the minimal inhibitory concentration of ethambutol. At times the patients fail to respond to treatment with anti tubercular drugs, the reasons for this could be many, one of them being drug resistance, which is increasingly being reported from both developed and developing countries Objectives: The present study was planned to establish a method for testing of an antitubercular agent i.e. Ethambutol is a narrowspectrum antimycobacterial agent that is used for the treatment of tuberculosis. Background: Tuberculosis has always been and still has the distinction of being a leading contender for one of the top places on the list of the main cause of death in India.
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